Phospholipase A1 of human blood platelets (Short Communication)

Phospholipase D in human blood platelets

The effect of phospholipase C on human blood platelets.

The effect of phospholipase C (EC 3.1.4.3) on human blood platelets has been studied. Phospholipase C from Bacillus cereus was purified to homogeneity as judged by analytical and sodium dodecyl sulphate disc gel electrophoresis and by immunoelectrophoresis. Human platelets isolated from platelet-rich plasma by gel filtration or by centrifugation and washing were incubated with phospholipase C. ...

Soybean Oil Degumming by Immobilized Phospholipase A1

In the present study, we investigated the ability of an immobilized phospholipase A1 (PLA1) in degumming of soybean oil. The enzyme was immobilized by simple adsorption on bentonite without any further modification. The free and immobilized PLA1 were characterized by Fourier Transform InfraRed (FT-IR) spectroscopy and X-Ray Diffraction (XRD). The immobilizat...

Trypsin-induced phospholipase activity in human platelets.

Trypsin mediates a release of arachidonic acid with resultant increase in O2 consumption (a reflection of cyclo-oxygenase activity) by whole human platelets that is similar to thrombin's effect on these cells. The trypsin and thrombin effects can be differentiated in two ways: (1) at saturating concentrations the measured effects of trypsin greatly exceed those of thrombin; (2) EGTA [ethanediox...

Plasmalogen in human blood platelets.

Plasmalogens have now been identified in a wide variety of organisms (l-3). In studies previously reported, plasmalogens were identified and roughly quantitated (4) in human platelet phosphatides. The present report concerns a precise determination of’plasmalogens in human blood platelets with the use of techniques not previously available. An evaluation of the possible role of plasmalogens in ...